65

QuantiChrom™ alpha-Glucosidase Assay Kit

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SKU:
65-DAGD-100-GEN
Availability:
In Stock
NULL888.40

Description

QuantiChrom™ alpha-Glucosidase Assay Kit

 

Application: For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism.

Key Features:

  • High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
  • Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
  • Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method: OD405nm

Samples: Biological

Species: All

Size: 100 tests

Detection Limit: 2 U/L

Shelf Life: 6 months

More Details:

α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery.

Gentaur Genprice QuantiChrom™ α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

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Additional Information

Application:
Quantitative determination of α-glucosidase activity by colorimetric (405nm) method
Procedure:
20 min
Size:
100 Tests
Detection limit:
2 U/L
Shelf life:
6 months
Shipping:
Ambient temperature
Storage:
-20°C.
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