QuantiChrom™ Nitric Oxide Assay Kit

Application: For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.

Key Features:

• Sensitive and accurate. Detection range 0.6 - 200 μM in 96-well plate.
• Rapid and reliable. Using an optimized VCl₃ reagent, the time required for reduction of nitrate (NO₃^-) to nitrite (NO₂^-) is 10 min at 60°C.
• Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.

Method: OD540nm

Samples: Plasma, serum, urine, tissue/cells and foods

Species: All

Size: 100 tests

Detection Limit: 0.6 μM

Shelf Life: 6 months

More Details:

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery.

Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO^2-/NO^3- as a measure for NO level. Gentaur Genprice QuantiChrom™ Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 min.