QuantiFluo™ Diamine Oxidase Assay Kit

Application: For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc)

Key Features:

• Fast and sensitive. Use of 10 μL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: FL530/585nm

Samples: Serum, Plasma

Species: All

Size: 100 tests

Detection Limit: 0.5 U/L

Shelf Life: 6 months

More Details:

DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.

Gentaur Genprice non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH₃ and H₂O₂. The generated H₂O₂ is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λex/em = 530/585 nm is directly proportional to the enzyme activity.

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