QuicKey Pro Rat ADP/Acrp30 (Adiponectin) ELISA Kit | E-OSEL-R0006

QuicKey Pro Rat ADP/Acrp30 (Adiponectin) ELISA Kit | E-OSEL-R0006 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 1.5h

Sensitivity: 6.43ng/mL

Detection Range: 31.25-1000ng/mL

UniProt ID: N/A

Target Name: ADP/Acrp30

Target Synonym: Adiponectin, GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Rat ADP/Acrp30 in samples. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed.

Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.