R³ IGF-1, human recombinant | 4216

IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. ’s recombinant human IGF-I is a N-terminus modified IGF-I with a Long R3 chain, which shows higher potency than regular human IGF-I in bioactivity assay. The recombinant IGF-I Long R3 was produced from E. coli using Animal Origin Free (AOF) components and therefore it is suitable for bioproduction also. The product can be provided as lyophilized form or liquid form, stable for at least two years.

4216 | R³ IGF-1 human recombinant DataSheet

Biomolecule/Target: R3 IGF-1

Synonyms: Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

Alternates names: CCN1, G1G1 protein

Taglines: Highly potent form of endocrine hormone IGF-1

NCBI Gene ID #: 3491

NCBI Gene Symbol: CYR61

Gene Source: Human

Accession #: O00622

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 g/ml.

Biological activity: Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 39.4 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 50 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQPCDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF QPNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEEWVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GSSLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRVTN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPVRFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGETF SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD