RAB3A Antibody [AMM02525G]

RAB3A Antibody [AMM02525G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H, M, Rat

Specificity: This RAB3A antibody is generated from a mouse immunized with a recombinant protein.

Cellular Localisation: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P63012}. Lysosome Cytoplasmic vesicle, secretory vesicle {ECO:0000250|UniProtKB:P63012} Cell projection, axon {ECO:0000250|UniProtKB:P63011}. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form. {ECO:0000250|UniProtKB:P63012}

Molecular Weight: 24984

Clone: Monoclonal

Gene Name: RAB3A

Gene ID: 5864

Function: Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity) . Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity) . Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity) . Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity) . Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790) . Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141) . Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity) . Interacts with MADD (via uDENN domain) ; the GTP-bound form is preferred for interaction (By similarity) .

Summary: Tissue Location: Specifically expressed in brain.

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, FC, IF

Dilution: IF--1:25 FC--1:25 WB--1:2000 WB--1:500

Synonyms: Ras-related protein Rab-3A, RAB3A