Rabbit anti-Goat IgG (H&L), HRP conjugated | AS09 605

Rabbit anti-Goat IgG (H&L), HRP conjugated | AS09 605 | Gentaur UK, US & Europe Distribution

Immunogen: Purified goat IgG, whole molecule

Host: Rabbit

Conjugation: HRP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified rabbit IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Related Products: AS09 612 | Rabbit anti-goat IgG (H&L) biotin conjugated, secondary antibodiesAS09 630 | Rabbit anti-goat IgG (H&L), ALP conjugated, secondary antibodiesCollection of secondary antibodies

Recommended Dilutions: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Goat IgG heavy and light chains on all goat immunoglobulins

Predicted Reactivity: N/A

Not reactive in: Non-immunoglobulin goat serum proteins based in immunoelectrophoresis

Additional Information: For blocking BSA and non-fat milk is recommended to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.

Background: Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.

Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: HRP (horse radish peroxidase)

Research Area: Immunoassays