Rabbit APOA1 (Apolipoprotein A1) ELISA Kit | ELK2025

Rabbit APOA1 (Apolipoprotein A1) ELISA Kit | ELK2025 | Gentaur UK, US & Europe Distribution

Alternative Names: Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

Species: Rabbit

Assay Type: Competitive Inhibition

Sensitivity: 10.53 ng/mL

Standard: 2000 ng/mL

Detection Range: 31.25-2000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Cardiovascular biology;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apolipoprotein A1 (APOA1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the OD of the samples to the standard curve.