Rabbit APOE (Apolipoprotein E) ELISA Kit | ELK2024

Rabbit APOE (Apolipoprotein E) ELISA Kit | ELK2024 | Gentaur UK, US & Europe Distribution

Alternative Names: Apo-E; AD2; Apoprotein; Alzheimer Disease 2 (E4-Associated, Late Onset

Species: Rabbit

Assay Type: Competitive Inhibition

Sensitivity: 182.5 pg/mL

Standard: 40000 pg/mL

Detection Range: 625-40000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Metabolic pathway;Cardiovascular biology;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apolipoprotein E (APOE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein E (APOE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the OD of the samples to the standard curve.