Rabbit HCII (Heparin Cofactor II) ELISA Kit | ELK2890

Rabbit HCII (Heparin Cofactor II) ELISA Kit | ELK2890 | Gentaur UK, US & Europe Distribution

Alternative Names: SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 1.03 U/L

Standard: 200 U/L

Detection Range: 3.13-200 U/L

Sample Type: Plasma.

Assay Lenght: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Cofactor II (HCII) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparin Cofactor II (HCII) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Cofactor II (HCII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Cofactor II (HCII) in the samples is then determined by comparing the OD of the samples to the standard curve.