Rabbit Proteolipid protein 2 (PLP2) ELISA Kit | AE26935RB

Rabbit Proteolipid protein 2 (PLP2) ELISA Kit | AE26935RB | Gentaur UK, US & Europe Distribution

Species Reactivity: Rabbit (Oryctolagus cuniculus)

Abbreviation: PLP2

Alternative Name: A4; A4-LSB; MGC126187; A4 differentiation-dependent protein

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.065 ng/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤6.2%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLP2 in samples. An antibody specific for PLP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PLP2 encodes an integral membrane protein that localizes to the endoplasmic reticulum in colonic epithelial cells. The encoded protein can multimerize and may function as an ion channel. A polymorphism in the promoter of this gene may be linked to an increased risk of X-linked mental retardation. A pseudogene of this gene is found on chromosome 5. Oliva et al. (1993) cloned a cDNA encoding the A4 differentiation-dependent protein from a human colon cell line. Fisher et al. (1997) identified the promoter and complete coding sequence of the A4 gene, which is organized into 5 exons spanning approximately 3.5 kb of DNA. The product of this gene is an integral membrane protein, containing 4 putative transmembrane domains that may play a role in cell differentiation in the intestinal epithelium.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .