Raf-1 (phospho Ser301) Antibody | 50-237

Raf-1 (phospho Ser301) Antibody | 50-237 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Bovine, Chicken, Frog

Homology: N/A

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1.

Research Area: Phospho-Specific

Tested Application: WB

Application: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 74

Validation: N/A

Isoform: N/A

Purification: Affinity Purified

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: N/A

Concentration: N/A

Storage Condition: Raf-1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996) . Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003) . Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.