RAF1, Active | 7726

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).

7726 | RAF1, Active DataSheet

Biomolecule/Target :

Synonyms: RAF, RAF proto-oncogene serine/threonine-protein kinase

Alternates names: RAF, RAF proto-oncogene serine/threonine-protein kinase , Proto-oncogene c-RAF

Taglines: A proto-oncogene

NCBI Gene ID #: 5894

NCBI Gene Symbol: RAF1

Gene Source:

Accession #: P04049

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGE: ≥85%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 63.0 kDa

Concentration: 5ug/50 ul

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans