Description
RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Biomolecule/Target :
Synonyms: RAF, RAF proto-oncogene serine/threonine-protein kinase
Alternates names: RAF, RAF proto-oncogene serine/threonine-protein kinase , Proto-oncogene c-RAF
Taglines: A proto-oncogene
NCBI Gene ID #: 5894
NCBI Gene Symbol: RAF1
Gene Source:
Accession #: P04049
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGE: ≥85%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 63.0 kDa
Concentration: 5ug/50 ul
Appearance: Liquid
Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
Additional Information
Storage Temperature: |
-80°C |
Shipping: |
dry ice |
Shelf Life: |
12 months |