RANTES Antibody [5B3-D5] | XP-5735-M

RANTES Antibody [5B3-D5] | XP-5735-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Monoclonal antibody against human RANTES designated as VL-1 was purified from ascites fluid on a Protein A affinity column.

Research Area: Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL) , a concentration of 5-7 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: 5B3-D5

Isotype: RANTES antibody was derived form BALB/c mice and belongs to the IgG2b isotype.

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: RANTES lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: Regulated upon activation normally T expressed and presumably secreted, CCL5, TCP228, SISd, D17S136E, SCYA5, C-C motif chemokine 5, EoCP, eoCP, RANTES, SIS-delta

User Note: Centrifuge vial prior to opening.

BACKGROUND: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV) . It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.