RASSF1A, human recombinant | P1114

RASSF1A is similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. This protein required for death receptor-dependent apoptosis. Recombinant human RASSF1A protein, fused to His-tag at N-terminus, was expressed in E.coli.

P1114 | RASSF1A human recombinant DataSheet

Biomolecule/Target: RASSF1

Synonyms: Ras association domain-containing protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21

Alternates names: Delta-like protein 4; Drosophila Delta homolog 4

Taglines: Potential tumor suppressor

NCBI Gene ID #: 54567

NCBI Gene Symbol: DLL-4

Gene Source: Human

Accession #: Q9NR61

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): The sDLL-4, when immobilized at concentrations > 1.5 g/mL will inhibit myogenesis in C2C12 cells.

Biological activity: The sDLL-4, when immobilized at concentrations > 1.5 g/mL will inhibit myogenesis in C2C12 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 54.3 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP