Rat 6-pyruvoyl tetrahydrobiopterin synthase (PTS) ELISA Kit | AE25022RA

Rat 6-pyruvoyl tetrahydrobiopterin synthase (PTS) ELISA Kit | AE25022RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: PTS

Alternative Name: FLJ97081; PTPS; 6-pyruvoyl tetrahydrobiopterin synthase|PTP synthase

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤5.3%

Inter-Assay: ≤7.5%

Recovery: 1, 08

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PTS in samples. An antibody specific for PTS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: In enzymology, a 6-pyruvoyltetrahydropterin synthase (PTPS) (EC 4.2.3.12) is an enzyme that catalyzes the following chemical reaction: 7, 8-Dihydroneopterin triphosphate {\displaystyle \rightleftharpoons } \rightleftharpoons 6-pyruvoyltetrahydropterin + triphosphate Hence, this enzyme has one substrate, 7, 8-Dihydroneopterin triphosphate, and two products, 6-pyruvoyltetrahydropterin and triphosphate.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .