Rat ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit | ELK8162

Rat ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit | ELK8162 | Gentaur UK, US & Europe Distribution

Alternative Names: RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26

Species: Rat

Assay Type: Sandwich

Sensitivity: 12.8 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the OD of the samples to the standard curve.