Rat Anti-Mouse Epigen Antibody | 103-M379

Rat Anti-Mouse Epigen Antibody | 103-M379 | Gentaur UK, US & Europe Distribution

Species: Anti-Mouse

Host / biotech: Rat

Comment: N/A

Label: N/A

Clone / Antibody feature: (#2L47)

Subcategory: Monoclonal Antibody

Category: Antibody

Synonyms: Esm1; ESM-1; AV004503; 0610042H23Rik

Isotype: IgG2

Application: WB

Detection Range: N/A

Species Reactivity/Cross reactivity: Mouse

Antigen: recombinant mouse protein Epigen

Description: Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily. Mouse Epigen cDNA encodes a 152 amino acid (aa) residue type I transmembrane precursor protein with a 23 aa putative signal peptide, an 86 aa extracellular domain, a 21 aa transmembrane domain and a 22 aa cytoplasmic domain. The extracellular domain (aa 24-109) contains a pattern of six cysteines typical of EGF family proteins. Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form. Cleavage of Epigen by TACE/ADAM17 has been demonstrated. The mature, shed form of mouse Epigen (aa 53-103) shares 92%, 96% and 90% aa sequence identity with human, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGFα and epiregulin. Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands. Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate. Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated-depletion.

Purity Confirmation: N/A

Endotoxin: N/A

Formulation: lyophilized

Storage Handling Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Reconstituation: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Molecular Weight: N/A

Lenght (aa): N/A

Protein Sequence: N/A

NCBI Gene ID: 71920