Rat APOB100 (Apolipoprotein B100) ELISA Kit | ELK2623

Rat APOB100 (Apolipoprotein B100) ELISA Kit | ELK2623 | Gentaur UK, US & Europe Distribution

Alternative Names: Apo-B100

Species: Rat

Assay Type: Sandwich

Sensitivity: 4.9 ng/mL

Standard: 800 ng/mL

Detection Range: 12.5-800 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B100 (APOB100) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein B100 (APOB100) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein B100 (APOB100), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein B100 (APOB100) in the samples is then determined by comparing the OD of the samples to the standard curve.