Rat Apolipoprotein B100 (APO-B100) ELISA Kit | AE59365RA

Rat Apolipoprotein B100 (APO-B100) ELISA Kit | AE59365RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: APOB

Alternative Name: FLDB; LDLCQ4; apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48

Application: ELISA

Range: 12.5-800 ng/mL

Sensitivity: 4.9 ng/mL

Intra-Assay: ≤6.8%

Inter-Assay: ≤11.6%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate APOB in samples. An antibody specific for APOB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Apolipoprotein B100 is a protein that plays a role in metabolism. It is a form of low density lipoprotein (LDL) . Apo-B levels correlate with the risk of coronary disease. The protein occurs in the plasma in 2 main isoforms, APOB48 and APOB100. The first is synthesized exclusively by the small intestine, the second by the liver. Both isoforms are coded by APOB and by a single mRNA transcript larger than 16 kb. . Apo-B100 is the major physiological ligand for the LDL receptor. Apo-B100 is a large monomeric protein, containing 4536 amino acids. Apo-B100 is synthesized in the liver and is required for the assembly of VLDL. It does not interchange between lipoprotein particles, as do the other apolipoproteins, and it is found in IDL and LDL after the removal of the Apo-A, E and C. Apo-B48 is present in chylomicrons and their remnants. It is essential for the intestinal absorption of dietary lipids.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .