Rat BMP7 (Bone Morphogenetic Protein 7) ELISA Kit | ELK2636

Rat BMP7 (Bone Morphogenetic Protein 7) ELISA Kit | ELK2636 | Gentaur UK, US & Europe Distribution

Alternative Names: OP1; Osteogenic Protein-1; Eptotermin alfa

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.8 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 7 (BMP7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 7 (BMP7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 7 (BMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 7 (BMP7) in the samples is then determined by comparing the OD of the samples to the standard curve.