Description
Rat CAMP (Cathelicidin Antimicrobial Peptide) ELISA Kit | ELK8207 | Gentaur UK, US & Europe Distribution
Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
Species: Rat
Assay Type: Sandwich
Sensitivity: 57 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the OD of the samples to the standard curve.