Rat CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit | KTE100433

Rat CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit | KTE100433 | Gentaur UK, US & Europe Distribution

Application: This Rat CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat CAMP-dependent protein kinase type II-alpha regulatory subunit microplate
• Rat CAMP-dependent protein kinase type II-alpha regulatory subunit standard
• Rat CAMP-dependent protein kinase type II-alpha regulatory subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRKAR2A. No significant cross-reactivity or interference between Rat PRKAR2A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR2a is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of PKA. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) .

Alternative Names: PRKAR2A; MGC3606; PKR2; PRKAR2; OTTHUMP00000210266; cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase; regulatory subunit alpha 2; protein kinase A; RII-alpha subunit

Search name: PRKAR2A; MGC3606; PKR2; PRKAR2; OTTHUMP00000210266; cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase; regulatory subunit alpha 2; protein kinase A; RII-alpha subunit

Tag: PRKAR2A