Rat CAT2 (Cationic Amino Acid Transporter 2) ELISA Kit | ELK6989

Rat CAT2 (Cationic Amino Acid Transporter 2) ELISA Kit | ELK6989 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC7A2; ATRC2; CAT-2; HCAT2; Low Affinity Cationic Amino Acid Transporter 2; Solute Carrier Family 7 Member 2, Cationic Amino Acid Transporter, y+ System

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cationic Amino Acid Transporter 2 (CAT2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cationic Amino Acid Transporter 2 (CAT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cationic Amino Acid Transporter 2 (CAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cationic Amino Acid Transporter 2 (CAT2) in the samples is then determined by comparing the OD of the samples to the standard curve.