Rat CD200R1 (CD200 Receptor 1) ELISA Kit | ELK8788

Rat CD200R1 (CD200 Receptor 1) ELISA Kit | ELK8788 | Gentaur UK, US & Europe Distribution

Alternative Names: OX2R; HCRTR2; CD200R; MOX2R; MOX2 Receptor; Cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Cell surface glycoprotein OX2 receptor 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.6 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD200 Receptor 1 (CD200R1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD200 Receptor 1 (CD200R1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD200 Receptor 1 (CD200R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD200 Receptor 1 (CD200R1) in the samples is then determined by comparing the OD of the samples to the standard curve.