Description
Rat CLDN16 (Claudin 16) ELISA Kit | ELK7269 | Gentaur UK, US & Europe Distribution
Alternative Names: PCLN1; HOMG3; Paracellin-1; Hypomagnesemia 3, With Hypercalciuria And Nephrocalcinosis
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.53 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 16 (CLDN16) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 16 (CLDN16) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 16 (CLDN16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 16 (CLDN16) in the samples is then determined by comparing the OD of the samples to the standard curve.