Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit | ELK1411

Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit | ELK1411 | Gentaur UK, US & Europe Distribution

Alternative Names: HCNTF

Species: Rat

Assay Type: Sandwich

Sensitivity: 16 pg/mL

Standard: 2500 pg/mL

Detection Range: 39.07-2500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ciliary Neurotrophic Factor (CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ciliary Neurotrophic Factor (CNTF) in the samples is then determined by comparing the OD of the samples to the standard curve.