Rat COL6a1 (Collagen Type VI Alpha 1) ELISA Kit | ELK6979

Rat COL6a1 (Collagen Type VI Alpha 1) ELISA Kit | ELK6979 | Gentaur UK, US & Europe Distribution

Alternative Names: COL6-A1; OPLL; Collagen Alpha-1 (VI) chain

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VI Alpha 1 (COL6a1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type VI Alpha 1 (COL6a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VI Alpha 1 (COL6a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type VI Alpha 1 (COL6a1) in the samples is then determined by comparing the OD of the samples to the standard curve.