Rat Collectrin (TMEM27) ELISA Kit | AE14359RA

Rat Collectrin (TMEM27) ELISA Kit | AE14359RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: TMEM27

Alternative Name: NX-17; NX17; 0610008J07Rik|collectrin|kidney-specific membrane protein

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.061 ng/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤9.9%

Recovery: 0, 93

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TMEM27 in samples. An antibody specific for TMEM27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM27 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TMEM27 encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. TMEM27 has an N-terminal signal sequence, followed by a noncatalytic extracellular domain, a transmembrane segment, and a cytosolic domain. The extracellular domain contains an O-glycosylation site and 2 N-glycosylation sites. Human TMEM27 shares 47.8% identity with the C-terminal end of ACE2, but it lacks an ACE2-like catalytic domain. Northern blot analysis of rat, mouse, and human tissues detected a transcript of about 1.8 kb exclusively in kidney.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .