Rat CORT (Cortistatin) ELISA Kit | ELK8674

Rat CORT (Cortistatin) ELISA Kit | ELK8674 | Gentaur UK, US & Europe Distribution

Alternative Names: CST14; CST17; CST29

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cortistatin (CORT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cortistatin (CORT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cortistatin (CORT) in the samples is then determined by comparing the OD of the samples to the standard curve.