Rat Creatine kinase M-type (CKM) ELISA Kit | KTE100984

Rat Creatine kinase M-type (CKM) ELISA Kit | KTE100984 | Gentaur UK, US & Europe Distribution

Application: This Rat Creatine kinase M-type (CKM) ELISA Kit employs a two-site sandwich ELISA to quantitate CKM in samples. An antibody specific for CKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CKM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Creatine kinase M-type microplate
• Rat Creatine kinase M-type standard
• Rat Creatine kinase M-type detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Creatine kinase M-type (CKM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CKM. No significant cross-reactivity or interference between Rat CKM and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CKM is a cytoplasmic enzyme involved in cellular energy homeostasis. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine and between phospho-creatine and ADP. Its functional entity is a MM-CK homodimer in striated (sarcomeric) skeletal and cardiac muscle.In heart, in addition to the MM-CK homodimer also the heterodimer consisting of one muscle (MM-CK) and one brain-type (MB-CK) subunit is expressed. This latter is an important serum marker for myocardial infarction, for it is released from damaged myocardial cells into the blood where it can be detected by clinical chemistry. Since no other organ than cardiac muscle expresses significant levels of MB-CK, this clinical test is still fairly reliable, together with troponin-T, for diagnosing a myocardial infarction.

Alternative Names: CKM; CKMM; M-CK; creatine kinase M chain; creatine kinase-M; muscle creatine kinase

Search name: CKM; CKMM; M-CK; creatine kinase M chain; creatine kinase-M; muscle creatine kinase

Tag: CKM