Description
Rat CUBN (Cubilin) ELISA Kit | ELK7401 | Gentaur UK, US & Europe Distribution
Alternative Names: IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Metabolic pathway;Nutrition metabolism;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cubilin (CUBN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cubilin (CUBN) in the samples is then determined by comparing the OD of the samples to the standard curve.