855

Rat CXCR3 (Chemokine C-X-C-Motif Receptor 3) ELISA Kit | ELK1523

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SKU:
855-ELK1523-GEN
zł3,528.00 - zł4,224.00

Description

Rat CXCR3 (Chemokine C-X-C-Motif Receptor 3) ELISA Kit | ELK1523 | Gentaur UK, US & Europe Distribution

Alternative Names: CD183; CKR-L2; CMKAR3; GPR9; IP10r; IP10-R; Mig-R; MigR; G Protein-Coupled Receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 3 (CXCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Receptor 3 (CXCR3) in the samples is then determined by comparing the OD of the samples to the standard curve.

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