Rat CYPB (Cyclophilin B) ELISA Kit | ELK2867

Rat CYPB (Cyclophilin B) ELISA Kit | ELK2867 | Gentaur UK, US & Europe Distribution

Alternative Names: PPIB; PPI-B; CYP-S1; CyP-B; SCYLP; PPIase B; Rotamase B; S-cyclophilin; Peptidylprolyl Isomerase B; Peptidyl-Prolyl Cis-Trans Isomerase B

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.33 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B (CYPB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclophilin B (CYPB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin B (CYPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclophilin B (CYPB) in the samples is then determined by comparing the OD of the samples to the standard curve.