Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit | KTE100788

Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit | KTE100788 | Gentaur UK, US & Europe Distribution

Application: This Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit employs a two-site sandwich ELISA to quantitate DCUN1D5 in samples. An antibody specific for DCUN1D5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCUN1D5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCUN1D5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCUN1D5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat DCN1-like protein 5 microplate
• Rat DCN1-like protein 5 standard
• Rat DCN1-like protein 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCUN1D5. No significant cross-reactivity or interference between Rat DCUN1D5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DCUN1D5 cDNA encoded a protein of 237 amino acids, which contained two domains associated with DNA repair and cell cycle regulation. Although no reported studies have clearly demonstrated DCUN1D5’s role in cancer, one report suggested that DCUN1D5 expression may be useful as a biomarker for cervical cancer. Because DNA damage and repair pathways played critical roles in the development of LSCC, a smokingrelated cancer, and because DCUN1D5 was found to be up-regulated in our patients with LSCC who had a tobacco history, that DCUN1D5 had a vital function involved in DNA damage response and repair in the carcinogenesis of LSCC.

Alternative Names: DCUN1D5; FLJ32431; FLJ37425; MGC2714; DCN1; defective in cullin neddylation 1; domain containing 5

Search name: DCUN1D5; FLJ32431; FLJ37425; MGC2714; DCN1; defective in cullin neddylation 1; domain containing 5

Tag: DCUN1D5