Rat DLD (Dihydrolipoyl Dehydrogenase) ELISA Kit | ELK8903

Rat DLD (Dihydrolipoyl Dehydrogenase) ELISA Kit | ELK8903 | Gentaur UK, US & Europe Distribution

Alternative Names: DLD; Diaphorase; DLD; DLDD; DLDH; GCSL; LAD; Lipoamide Dehydrogenase; PHE3; 2-oxo-glutaRate complex; branched chain keto acid dehydrogenase complex; branched chain keto acid dehydrogenase complex; dihydrolipoamide dehydrogenase; E3 component of pyruvate dehydrogenase complex; dihydrolipoamide dehydrogenasemitochondrial; DLDH; E3; E3 component of pyruvate dehydrogenase complex; 2-oxo-glutaRate complex; EC 1.8.1; EC 1.8.1.4; GCSL; Glycine cleavage system L protein; glycine cleavage system protein L; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrolipoyl Dehydrogenase (DLD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dihydrolipoyl Dehydrogenase (DLD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrolipoyl Dehydrogenase (DLD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydrolipoyl Dehydrogenase (DLD) in the samples is then determined by comparing the OD of the samples to the standard curve.