Rat Galanin (GAL) ELISA Kit | KTE100990

Rat Galanin (GAL) ELISA Kit | KTE100990 | Gentaur UK, US & Europe Distribution

Application: This Rat Galanin (GAL) ELISA Kit employs a two-site sandwich ELISA to quantitate GAL in samples. An antibody specific for GAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Galanin microplate
• Rat Galanin standard
• Rat Galanin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Galanin (GAL) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GAL. No significant cross-reactivity or interference between Rat GAL and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Galanin is formed by the cleavage of a prepropeptide encoded by a gene known as GAL. It is involved in a number of physiological processes such as regulation of food intake, metabolism and reproduction, regulation of neurotransmitter and hormone release, nociception, intestinal contraction and secretion, and more recently in nervous system development and response to injury. This wide diversity of action is mediated by several galanin receptor subtypes.Galanin is predominantly an inhibitory, hyperpolarizing neuropeptide and as such inhibits neurotransmitter release. Galanin is often co-localized with classical neurotransmitters such as acetylcholine, serotonin and norepinephrine and also with other neuromodulators such as Neuropeptide Y, Substance P and Vasoactive intestinal peptide.

Alternative Names: GAL; GALN; GLNN; GMAP; MGC40167; galanin; galanin-message-associated peptide; galanin-related peptide

Search name: GAL; GALN; GLNN; GMAP; MGC40167; galanin; galanin-message-associated peptide; galanin-related peptide

Tag: GAL