Rat GHRL (Ghrelin) ELISA Kit | ELK6912

Rat GHRL (Ghrelin) ELISA Kit | ELK6912 | Gentaur UK, US & Europe Distribution

Alternative Names: MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 10.18 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin (GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin (GHRL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ghrelin (GHRL) in the samples is then determined by comparing the OD of the samples to the standard curve.