Rat HbA1c (Glycated Hemoglobin A1c) ELISA Kit | ELK5370

Rat HbA1c (Glycated Hemoglobin A1c) ELISA Kit | ELK5370 | Gentaur UK, US & Europe Distribution

Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 4.25 μg/mL

Standard: 1000 μg/mL

Detection Range: 15.63-1000 μg/mL

Sample Type: plasma and erythrocyte lysates

Assay Lenght: 2h

Research Area: Diagnosis of diabetes mellitus

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Hemoglobin A1c (HbA1c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c (HbA1c) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycated Hemoglobin A1c (HbA1c) in the samples is then determined by comparing the OD of the samples to the standard curve.