Rat Heat shock factor-binding protein 1 (HSBP1) ELISA Kit | AE60834RA

Rat Heat shock factor-binding protein 1 (HSBP1) ELISA Kit | AE60834RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: HSBP1

Alternative Name: DKFZp686D1664; DKFZp686O24200; NPC-A-13;

Application: ELISA

Range: 78.12-5000 pg/mL

Sensitivity: 30 pg/mL

Intra-Assay: ≤4.2%

Inter-Assay: ≤9.6%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate HSBP1 in samples. An antibody specific for HSBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .