Rat High affinity choline transporter 1 (SLC5A7) ELISA Kit | KTE100247

Rat High affinity choline transporter 1 (SLC5A7) ELISA Kit | KTE100247 | Gentaur UK, US & Europe Distribution

Application: This Rat High affinity choline transporter 1 (SLC5A7) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A7 in samples. An antibody specific for SLC5A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat High affinity choline transporter 1 microplate
• Rat High affinity choline transporter 1 standard
• Rat High affinity choline transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat High affinity choline transporter 1 (SLC5A7) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC5A7. No significant cross-reactivity or interference between Rat SLC5A7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Choline is a direct precursor of acetylcholine (ACh), a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. SLC5A7 is a Na (+) - and Cl (-) - dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons.The deduced 580-amino acid protein has a calculated molecular mass of 63.2 kD. CHT has 13 transmembrane (TM) domains, a short extracellular N terminus, and a long cytoplasmic C terminus. It contains 3 putative N-glycosylation sites and several phosphorylation sites. CHT shares 93% amino acid identity with rat Cht and 51% amino acid identity with its nematode homolog, Cho1.

Alternative Names: SLC5A7; CHT; CHT1; MGC126299; MGC126300; hCHT; OTTHUMP00000203760; high affinity choline transporter; hemicholinium-3-sensitive choline transporter

Search name: SLC5A7; CHT; CHT1; MGC126299; MGC126300; hCHT; OTTHUMP00000203760; high affinity choline transporter; hemicholinium-3-sensitive choline transporter

Tag: SLC5A7