Rat Leukosialin (SPN) ELISA Kit | AE14988RA

Rat Leukosialin (SPN) ELISA Kit | AE14988RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: SPN

Alternative Name: CD43; GPL115; LSN; leukosialin|sialophorin (gpL115; leukosialin; CD43) |sialophorin (leukosialin; CD43)

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.3%

Inter-Assay: ≤9.7%

Recovery: 0, 88

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SPN in samples. An antibody specific for SPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .