Rat Long-chain fatty acid transport protein 1 (SLC27A1) ELISA Kit | AE18838RA

Rat Long-chain fatty acid transport protein 1 (SLC27A1) ELISA Kit | AE18838RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: SLC27A1

Alternative Name: ACSVL5; FATP; FATP1; FLJ00336; MGC71751; solute carrier family 27; member 1

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.058 ng/mL

Intra-Assay: ≤5.4%

Inter-Assay: ≤11.4%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC27A1 in samples. An antibody specific for SLC27A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC27A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC27A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC27A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Transfected cells were grown in the presence of a fluorescent fatty acid analog and those that became positive for the dye were sorted and the cDNA recovered. Two classes of clones were identified: one encodes a 699-amino acid protein resembling rat liver fatty acyl CoA synthetase (FACL2), and the second encodes a 646-amino acid protein with 6 potential transmembrane domains. Northern blot analysis showed high levels of FATP in 3T3-L1 adipocytes, with a major 2.9-kb transcript and a minor 3.8-kb form. Expression increased 5-fold during differentiation of preadipocytes to adipocytes. In normal mouse tissues, FATP mRNA levels were highest in skeletal muscle, heart, and fat. Expression studies showed that FATP increased the uptake of labeled oleic acid by 3- to 4-fold, and similar effects were observed with other LCFAs.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .