Rat Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit | KTE100578

Rat Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit | KTE100578 | Gentaur UK, US & Europe Distribution

Application: This Rat Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit employs a two-site sandwich ELISA to quantitate MSRA in samples. An antibody specific for MSRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMSRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MSRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MSRA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Mitochondrial peptide methionine sulfoxide reductase microplate
• Rat Mitochondrial peptide methionine sulfoxide reductase standard
• Rat Mitochondrial peptide methionine sulfoxide reductase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MSRA. No significant cross-reactivity or interference between Rat MSRA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MSRA encodes a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein functions in the repair of oxidatively damaged proteins to restore biological activity. Alternative splicing results in multiple transcript variants.

Alternative Names: MSRA; Cytosolic methionine-S-sulfoxide reductase; peptide met (O) reductase

Search name: MSRA; Cytosolic methionine-S-sulfoxide reductase; peptide met (O) reductase

Tag: MSRA