Rat Muscarinic acetylcholine receptor M2 (CHRM2) ELISA Kit | KTE100905

Rat Muscarinic acetylcholine receptor M2 (CHRM2) ELISA Kit | KTE100905 | Gentaur UK, US & Europe Distribution

Application: This Rat Muscarinic acetylcholine receptor M2 (CHRM2) ELISA Kit employs a two-site sandwich ELISA to quantitate CHRM2 in samples. An antibody specific for CHRM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Muscarinic acetylcholine receptor M2 microplate
• Rat Muscarinic acetylcholine receptor M2 standard
• Rat Muscarinic acetylcholine receptor M2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Muscarinic acetylcholine receptor M2 (CHRM2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CHRM2. No significant cross-reactivity or interference between Rat CHRM2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: He is a muscarinic acetylcholine receptor.M2 muscarinic receptors act via a Gi type receptor, which causes a decrease in cAMP in the cell, generally leading to inhibitory-type effects.In addition, they modulate muscarinic potassium channels. In the heart, this contributes to a decreased heart rate.The M2 muscarinic receptors are located in the heart, where they act to slow the heart rate down to normal sinus rhythm after stimulatory actions of the sympathetic nervous system, by slowing the speed of depolarization. They also reduce contractile forces of the atrial cardiac muscle, and reduce conduction velocity of the atrioventricular node (AV node) . However, they have no effect on the contractile forces of the ventricular muscle.

Alternative Names: CHRM2; FLJ43243; HM2; MGC120006; MGC120007; 7TM receptor; OTTHUMP00000208394; OTTHUMP00000208395; cholinergic receptor; muscarinic 2; isoform a; muscarinic M2 receptor; muscarinic acetylcholine receptor M2

Search name: CHRM2; FLJ43243; HM2; MGC120006; MGC120007; 7TM receptor; OTTHUMP00000208394; OTTHUMP00000208395; cholinergic receptor; muscarinic 2; isoform a; muscarinic M2 receptor; muscarinic acetylcholine receptor M2

Tag: CHRM2