Rat MyoD (Myogenic Differentiation) ELISA Kit | ELK6019

Rat MyoD (Myogenic Differentiation) ELISA Kit | ELK6019 | Gentaur UK, US & Europe Distribution

Alternative Names: MYOD1; PUM; MYF3; bHLHc1; Myoblast Determination Protein 1; Myogenic Factor 3; Class C basic helix-loop-helix protein 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology;Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myogenic Differentiation (MyoD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myogenic Differentiation (MyoD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myogenic Differentiation (MyoD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myogenic Differentiation (MyoD) in the samples is then determined by comparing the OD of the samples to the standard curve.