Rat NPY (Neuropeptide Y) ELISA Kit | ELK2448

Rat NPY (Neuropeptide Y) ELISA Kit | ELK2448 | Gentaur UK, US & Europe Distribution

Alternative Names: NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.94 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y (NPY) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropeptide Y (NPY) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y (NPY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide Y (NPY) in the samples is then determined by comparing the OD of the samples to the standard curve.