Rat NRP2 (Neuropilin 2) ELISA Kit | ELK7760

Rat NRP2 (Neuropilin 2) ELISA Kit | ELK7760 | Gentaur UK, US & Europe Distribution

Alternative Names: NP2; NPN2; VEGF165R2; VEGF165-R2; Vascular endothelial cell growth factor 165 receptor 2

Species: Rat

Assay Type: Sandwich

Sensitivity: 29.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin 2 (NRP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropilin 2 (NRP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropilin 2 (NRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropilin 2 (NRP2) in the samples is then determined by comparing the OD of the samples to the standard curve.