855
Rat PCSK1 (Proprotein Convertase Subtilisin/Kexin Type 1) ELISA Kit | ELK6822
- SKU:
- 855-ELK6822-GEN
Description
Rat PCSK1 (Proprotein Convertase Subtilisin/Kexin Type 1) ELISA Kit | ELK6822 | Gentaur UK, US & Europe Distribution
Alternative Names: PC1, PC3, SPC3; NEC1; Prohormone Convertase 3; Prohormone Convertase 1; Neuroendocrine Convertase 1; Proprotein Convertase 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 1.44 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the OD of the samples to the standard curve.